Application

Research Sub CategoryWhole Immunoglobulin Secondary Antibodies

Suggested dilution for most applications: 1:50-1:200 for most applications.Optimal working dilutions must be determined by the end user.

This Rabbit anti-Goat IgG Antibody, Rhodamine conjugate is validated for use in IF for the detection of Goat IgG.

Research CategorySecondary & Control Antibodies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, 0.01% Thimerosal and 0.05% Sodium Azide.

RECONSTITUTION:

Reconstitute to 1 mg/mL with 1.5mL sterile distilled water.

Specificity

Goat IgG (H+L)

FLUOROPHORE/PROTEIN ABSORBANCE RATIO

Tetramethyl Rhodamine Isothiocyanate A550/A280 = 0.46

The antibody is isolated from antisera by immunoaffinity chromatography using antigen coupled to agarose beads. No antibody was detected against non-immunoglobulin serum proteins by ELISA, antibodies may react with immunoglobulines from some other species.

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.